Cotton Cultivar L-9009-6

ABSTRACT

A cotton cultivar, designated L-9009-6, is disclosed. The invention relates to the seeds of cotton cultivar L-9009-6, to the plants of cotton L-9009-6 and to methods for producing a cotton plant produced by crossing the cultivar L-9009-6 with itself or another cotton variety. The invention further relates to hybrid cotton seeds and plants produced by crossing the cultivar L-9009-6 with another cotton cultivar.

BACKGROUND OF THE INVENTION

The present invention relates to a cotton (Gossypium barbadense L.)seed, a cotton plant, a cotton cultivar, and a cotton hybrid. Thisinvention further relates to a method for producing cotton seed andplants. All publications cited in this application are hereinincorporated by reference.

There are numerous steps in the development of any novel, desirableplant germplasm. Plant breeding begins with the analysis and definitionof problems and weaknesses of the current germplasm, the establishmentof program goals, and the definition of specific breeding objectives.The next step is selection of germplasm that possess the traits to meetthe program goals. The goal is to combine in a single cultivar animproved combination of desirable traits from the parental germplasm. Incotton, the important traits include higher fiber (lint) yield, earliermaturity, improved fiber quality, resistance to diseases and insects,resistance to drought and heat, and improved agronomic traits.

Choice of breeding or selection methods depends on the mode of plantreproduction, the heritability of the trait(s) being improved, and thetype of cultivar used commercially (e.g., F₁ hybrid cultivar, purelinecultivar, etc.). For highly heritable traits, a choice of superiorindividual plants evaluated at a single location will be effective,whereas for traits with low heritability, selection should be based onmean values obtained from replicated evaluations of families of relatedplants. Popular selection methods commonly include pedigree selection,modified pedigree selection, mass selection, and recurrent selection.

The complexity of inheritance influences choice of the breeding method.Backcross breeding is used to transfer one or a few favorable genes fora highly heritable trait into a desirable cultivar. This approach hasbeen used extensively for breeding disease-resistant cultivars. Variousrecurrent selection techniques are used to improve quantitativelyinherited traits controlled by numerous genes. The use of recurrentselection in self-pollinating crops depends on the ease of pollination,the frequency of successful hybrids from each pollination, and thenumber of hybrid offspring from each successful cross.

Each breeding program should include a periodic, objective evaluation ofthe efficiency of the breeding procedure. Evaluation criteria varydepending on the goal and objectives, but should include gain fromselection per year based on comparisons to an appropriate standard,overall value of the advanced breeding lines, and number of successfulcultivars produced per unit of input (e.g., per year, per dollarexpended, etc.).

Promising advanced breeding lines are thoroughly tested and compared topopular cultivars in environments representative of the commercialtarget area(s) for three or more years. The best lines havingsuperiority over the popular cultivars are candidates to become newcommercial cultivars. Those lines still deficient in a few traits arediscarded or utilized as parents to produce new populations for furtherselection.

These processes, which lead to the final step of marketing anddistribution, usually take from seven to twelve years from the time thefirst cross is made. Therefore, development of new cultivars is atime-consuming process that requires precise forward planning, efficientuse of resources, and a minimum of changes in direction.

A most difficult task is the identification of individuals that aregenetically superior because for most traits the true genotypic value ismasked by other confounding plant traits or environmental factors. Onemethod of identifying a superior plant is to observe its performancerelative to other experimental lines and widely grown standardcultivars. For many traits a single observation is inconclusive, andreplicated observations over time and space are required to provide agood estimate of a line's genetic worth.

The goal of a commercial cotton breeding program is to develop new,unique, and superior cotton cultivars. The breeder initially selects andcrosses two or more parental lines, followed by generation advancementand selection, thus producing many new genetic combinations. The breedercan theoretically generate billions of different genetic combinationsvia this procedure. The breeder has no direct control over which geneticcombinations will arise in the limited population size which is grown.Therefore, two breeders will never develop the same line having the sametraits.

Each year, the plant breeder selects the germplasm to advance to thenext generation. This germplasm is grown under unique and differentgeographical, climatic, and soil conditions, and further selections arethen made, during and at the end of the growing season. The lines whichare developed are unpredictable. This unpredictability is because thebreeder's selection occurs in unique environments, with no control atthe DNA level (using conventional breeding procedures), and withmillions of different possible genetic combinations being generated. Abreeder of ordinary skill in the art cannot predict the final resultinglines he develops, except possibly in a very gross and general fashion.The same breeder cannot produce, with any reasonable likelihood, thesame cultivar twice by using the exact same original parents and thesame selection techniques. This unpredictability results in theexpenditure of large amounts of research moneys to develop superior newcotton cultivars.

Pureline cultivars of cotton are commonly bred by hybridization of twoor more parents followed by selection. The complexity of inheritance,the breeding objectives, and the available resources influence thebreeding method. Pedigree breeding, recurrent selection breeding, andbackcross breeding are breeding methods commonly used in self pollinatedcrops such as cotton. These methods refer to the manner in whichbreeding pools or populations are made in order to combine desirabletraits from two or more cultivars or various broad-based sources. Theprocedures commonly used for selection of desirable individuals orpopulations of individuals are called mass selection, plant-to-rowselection, and single seed descent or modified single seed descent. One,or a combination of, these selection methods can be used in thedevelopment of a cultivar from a breeding population.

Pedigree breeding is primarily used to combine favorable genes into atotally new cultivar that is different in many traits than either parentused in the original cross. It is commonly used for the improvement ofself-pollinating crops. Two parents which possess favorable,complementary traits are crossed to produce an F₁ (filial generation 1).An F₂ population is produced by selfing F₁ plants. Selection ofdesirable individual plants may begin as early as the F₂ generationwherein maximum gene segregation occurs. Individual plant selection canoccur for one or more generations. Successively, seed from each selectedplant can be planted in individual, identified rows or hills, known asprogeny rows or progeny hills, to evaluate the line and to increase theseed quantity, or to further select individual plants. Once a progenyrow or progeny hill is selected as having desirable traits, it becomeswhat is known as a breeding line that is specifically identifiable fromother breeding lines that were derived from the same originalpopulation. At an advanced generation (i.e., F₅ or higher) seed ofindividual lines are evaluated in replicated testing. At an advancedstage the best lines or a mixture of phenotypically similar lines fromthe same original cross are tested for potential release as newcultivars.

The single seed descent procedure in the strict sense refers to plantinga segregating population, harvesting one seed from every plant, andcombining these seeds into a bulk which is planted the next generation.When the population has been advanced to the desired level ofinbreeding, the plants from which lines are derived will each trace todifferent F₂ individuals. Primary advantages of the seed descentprocedures are to delay selection until a high level of homozygosity(e.g., lack of gene segregation) is achieved in individual plants, andto move through these early generations quickly, usually through usingwinter nurseries.

The modified single seed descent procedures involve harvesting multipleseed (i.e., a single lock or a simple boll) from each plant in apopulation and combining them to form a bulk. Part of the bulk is usedto plant the next generation and part is put in reserve. This procedurehas been used to save labor at harvest and to maintain adequate seedquantities of the population.

Selection for desirable traits can occur at any segregating generation(F₂ and above). Selection pressure is exerted on a population by growingthe population in an environment where the desired trait is maximallyexpressed and the individuals or lines possessing the trait can beidentified. For instance, selection can occur for disease resistancewhen the plants or lines are grown in natural or artificially-induceddisease environments, and the breeder selects only those individualshaving little or no disease and are thus assumed to be resistant.

In addition to phenotypic observations, the genotype of a plant can alsobe examined. There are many laboratory-based techniques available forthe analysis, comparison, and characterization of plant genotype. Amongthese are Isozyme Electrophoresis, Restriction Fragment LengthPolymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs),Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Amplified Fragment Length polymorphisms (AFLPs), Simple Sequence Repeats(SSRs—which are also referred to as Microsatellites), and SingleNucleotide Polymorphisms (SNPs).

Isozyme Electrophoresis and RFLPs have been widely used to determinegenetic composition. Shoemaker and Olsen, (Molecular Linkage Map ofSoybean (Glycine max L. Merr.) pp. 6.131-6.138 in S. J. O'Brien (Ed.)Genetic Maps: Locus Maps of Complex Genomes, Cold Spring HarborLaboratory Press, Cold Spring Harbor, N.Y., (1993)) developed amolecular genetic linkage map that consisted of 25 linkage groups withabout 365 RFLP, 11 RAPD, three classical markers, and four isozyme loci.See also, Shoemaker, R. C., RFLP Map of Soybean, pp. 299-309, inPhillips, R. L. and Vasil, I. K. (Eds.), DNA-Based Markers in Plants,Kluwer Academic Press, Dordrecht, the Netherlands (1994).

SSR technology is currently the most efficient and practical markertechnology; more marker loci can be routinely used and more alleles permarker locus can be found using SSRs in comparison to RFLPs. Forexample, Diwan and Cregan described a highly polymorphic microsatellitelocus in soybean with as many as 26 alleles. Diwan, N. and Cregan, P.B., Theor. Appl. Genet., 95:22-225 (1997). SNPs may also be used toidentify the unique genetic composition of the invention and progenyvarieties retaining that unique genetic composition. Various molecularmarker techniques may be used in combination to enhance overallresolution.

Molecular markers, which includes markers identified through the use oftechniques such as Isozyme Electrophoresis, RFLPs, RAPDs, AP-PCR, DAF,SCARs, AFLPs, SSRs, and SNPs, may be used in plant breeding. One use ofmolecular markers is Quantitative Trait Loci (QTL) mapping. QTL mappingis the use of markers which are known to be closely linked to allelesthat have measurable effects on a quantitative trait. Selection in thebreeding process is based upon the accumulation of markers linked to thepositive effecting alleles and/or the elimination of the markers linkedto the negative effecting alleles from the plant's genome.

Molecular markers can also be used during the breeding process for theselection of qualitative traits. For example, markers closely linked toalleles or markers containing sequences within the actual alleles ofinterest can be used to select plants that contain the alleles ofinterest during a backcrossing breeding program. For example, molecularmarkers are used in soybean breeding for selection of the trait ofresistance to soybean cyst nematode, see U.S. Pat. No. 6,162,967. Themarkers can also be used to select toward the genome of the recurrentparent and against the markers of the donor parent. Using this procedurecan attempt to minimize the amount of genome from the donor parent thatremains in the selected plants. It can also be used to reduce the numberof crosses back to the recurrent parent needed in a backcrossingprogram. The use of molecular markers in the selection process is oftencalled Genetic Marker Enhanced Selection. Molecular markers may also beused to identify and exclude certain sources of germplasm as parentalvarieties or ancestors of a plant by providing a means of trackinggenetic profiles through crosses as discussed more fully hereinafter.

Mutation breeding is another method of introducing new traits intocotton varieties. Mutations that occur spontaneously or are artificiallyinduced can be useful sources of variability for a plant breeder. Thegoal of artificial mutagenesis is to increase the rate of mutation for adesired characteristic. Mutation rates can be increased by manydifferent means including temperature, long-term seed storage, tissueculture conditions, radiation (such as X-rays, Gamma rays, neutrons,Beta radiation, or ultraviolet radiation), chemical mutagens (such asbase analogues like 5-bromo-uracil), antibiotics, alkylating agents(such as sulfur mustards, nitrogen mustards, epoxides, ethyleneamines,sulfates, sulfonates, sulfones, or lactones), azide, hydroxylamine,nitrous acid, or acridines. Once a desired trait is observed throughmutagenesis the trait may then be incorporated into existing germplasmby traditional breeding techniques. Details of mutation breeding can befound in Principles of Cultivar Development by Fehr, MacmillanPublishing Company (1993).

The production of double haploids can also be used for the developmentof homozygous varieties in a breeding program. Double haploids areproduced by the doubling of a set of chromosomes from a heterozygousplant to produce a completely homozygous individual. For example, seeWan, et al., Theor. Appl. Genet., 77:889-892 (1989).

Descriptions of other breeding methods that are commonly used fordifferent traits and crops can be found in one of several referencebooks (e.g., Allard (1960); Simmonds (1979); Sneep, et al. (1979); Fehr(1987)).

Proper testing should detect any major faults and establish the level ofsuperiority or improvement over current cultivars. In addition toshowing superior performance, there must be a demand for a new cultivarthat is compatible with industry standards or which creates a newmarket. The introduction of a new cultivar will incur additional coststo the seed producer, and to the grower, processor, and consumer, forspecial advertising, marketing and commercial production practices, andnew product utilization. The testing preceding the release of a newcultivar should take into consideration research and development costsas well as technical superiority of the final cultivar. Forseed-propagated cultivars, it must be feasible to produce seed easilyand economically.

Cotton, including Gossypium hirsutum (Acala) and Gossypium barbadense(Pima), is an important and valuable field crop. Thus, a continuing goalof cotton plant breeders is to develop stable, high yielding cottoncultivars of both cotton species that are agronomically sound. Thereasons for this goal are obviously to maximize the amount and qualityof the fiber produced on the land used and to supply fiber, oil, andfood for animals and humans. To accomplish this goal, the cotton breedermust select and develop plants that have the traits that result insuperior cultivars.

The development of new cotton cultivars requires the evaluation andselection of parents and the crossing of these parents. The lack ofpredictable success of a given cross requires that a breeder, in anygiven year, make several crosses with the same or different breedingobjectives.

The cotton flower is monoecious in that the male and female structuresare in the same flower. The crossed or hybrid seed is produced by manualcrosses between selected parents. Floral buds of the parent that is tobe the female are emasculated prior to the opening of the flower bymanual removal of the male anthers. At flowering, the pollen fromflowers of the parent plants designated as male, are manually placed onthe stigma of the previous emasculated flower. Seed developed from thecross is known as first generation (F₁) hybrid seed. Planting of thisseed produces F₁ hybrid plants of which half their genetic component isfrom the female parent and half from the male parent. Segregation ofgenes begins at meiosis thus producing second generation (F₂) seed.Assuming multiple genetic differences between the original parents, eachF₂ seed has a unique combination of genes.

The foregoing examples of the related art and limitations relatedtherewith are intended to be illustrative and not exclusive. Otherlimitations of the related art will become apparent to those of skill inthe art upon a reading of the specification.

SUMMARY OF THE INVENTION

The following embodiments and aspects thereof are described inconjunction with systems, tools, and methods which are meant to beexemplary and illustrative, not limiting in scope. In variousembodiments, one or more of the above-described problems have beenreduced or eliminated, while other embodiments are directed to otherimprovements.

The present invention relates to a cotton seed, a cotton plant, a cottoncultivar, and a method for producing a cotton plant.

The present invention further relates to a method of producing cottonseeds and plants by crossing a plant of the instant invention withanother cotton plant.

One aspect of the present invention relates to seed of the cottonvariety L-9009-6. The invention also relates to plants produced bygrowing the seed of the cotton variety L-9009-6, as well as thederivatives of such plants. As used herein, the term “plant” includesplant cells, plant protoplasts, plant cells of a tissue culture fromwhich cotton plants can be regenerated, plant calli, plant clumps, andplant cells that are intact in plants or parts of plants, such aspollen, flowers, seeds, bolls, leaves, stems, and the like.

Another aspect of the invention relates to a tissue culture ofregenerable cells of the cotton variety L-9009-6, as well as plantsregenerated therefrom, wherein the regenerated cotton plant expressesall the physiological and morphological characteristics of a plant grownfrom the cotton seed designated L-9009-6.

Yet another aspect of the current invention is a cotton plant of thecotton variety L-9009-6 comprising at least a first transgene, whereinthe cotton plant is otherwise capable of expressing all thephysiological and morphological characteristics of the cotton varietyL-9009-6. In particular embodiments of the invention, a plant isprovided that comprises a single locus conversion. A single locusconversion may comprise a transgenic gene which has been introduced bygenetic transformation into the cotton variety L-9009-6 or a progenitorthereof. A transgenic or non-transgenic single locus conversion can alsobe introduced by backcrossing, as is well known in the art. In certainembodiments of the invention, the single locus conversion may comprise adominant or recessive allele. The locus conversion may conferpotentially any desired trait upon the plant as described herein.

Still yet another aspect of the invention relates to a first generation(F₁) hybrid cotton seed produced by crossing a plant of the cottonvariety L-9009-6 to a second cotton plant. Also included in theinvention are the F₁ hybrid cotton plants grown from the hybrid seedproduced by crossing the cotton variety L-9009-6 to a second cottonplant. Still further included in the invention are the seeds of an F₁hybrid plant produced with the cotton variety L-9009-6 as one parent,the second generation (F₂) hybrid cotton plant grown from the seed ofthe F₁ hybrid plant, and the seeds of the F₂ hybrid plant.

Still yet another aspect of the invention is a method of producingcotton seeds comprising crossing a plant of the cotton variety L-9009-6to any second cotton plant, including itself or another plant of thevariety L-9009-6. In particular embodiments of the invention, the methodof crossing comprises the steps of: (a) planting seeds of the cottonvariety L-9009-6; (b) cultivating cotton plants resulting from saidseeds until said plants bear flowers; (c) allowing fertilization of theflowers of said plants; and (d) harvesting seeds produced from saidplants.

Still yet another aspect of the invention is a method of producinghybrid cotton seeds comprising crossing the cotton variety L-9009-6 to asecond, distinct cotton plant which is nonisogenic to the cotton varietyL-9009-6. In particular embodiments of the invention, the crossingcomprises the steps of: (a) planting seeds of cotton variety L-9009-6and a second, distinct cotton plant; (b) cultivating the cotton plantsgrown from the seeds until the plants bear flowers; (c) crosspollinating a flower on one of the two plants with the pollen of theother plant; and (d) harvesting the seeds resulting from the crosspollinating.

Still yet another aspect of the invention is a method for developing acotton plant in a cotton breeding program comprising: obtaining a cottonplant, or its parts, of the variety L-9009-6; and b) employing saidplant or parts as a source of breeding material using plant breedingtechniques. In the method, the plant breeding techniques may be selectedfrom the group consisting of recurrent selection, mass selection, bulkselection, backcrossing, pedigree breeding, genetic marker-assistedselection, and genetic transformation. In certain embodiments of theinvention, the cotton plant of variety L-9009-6 is used as the male orfemale parent.

Still yet another aspect of the invention is a method of producing acotton plant derived from the cotton variety L-9009-6, the methodcomprising the steps of: (a) preparing a progeny plant derived fromcotton variety L-9009-6 by crossing a plant of the cotton varietyL-9009-6 with a second cotton plant; and (b) crossing the progeny plantwith itself or a second plant to produce a progeny plant of a subsequentgeneration which is derived from a plant of the cotton variety L-9009-6.In one embodiment of the invention, the method further comprises: (c)crossing the progeny plant of a subsequent generation with itself or asecond plant; and (d) repeating steps (b) and (c) for at least 2-10additional generations to produce an inbred cotton plant derived fromthe cotton variety L-9009-6. Also provided by the invention is a plantproduced by this and the other methods of the invention. Plant varietyL-9009-6-derived plants produced by this and the other methods of theinvention described herein may, in certain embodiments of the invention,be further defined as comprising the traits of plant variety L-9009-6given in Table 1.

The use of the word “a” or “an” when used in conjunction with the term“comprising” in the claims and/or the specification may mean “one,” butit is also consistent with the meaning of “one or more,” “at least one,”and “one or more than one.”

Other objects, features, and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and the specificexamples, while indicating specific embodiments of the invention, aregiven by way of illustration only, since various changes andmodifications within the spirit and scope of the invention will becomeapparent to those skilled in the art from this detailed description.

In another aspect, the present invention provides regenerable cells foruse in tissue culture of cotton plant L-9009-6. The tissue culture willpreferably be capable of regenerating plants having the physiologicaland morphological characteristics of the foregoing cotton plant, and ofregenerating plants having substantially the same genotype as theforegoing cotton plant. Preferably, the regenerable cells in such tissuecultures will be embryos, protoplasts, meristematic cells, callus,pollen, leaves, anthers, pistils, roots, root tips, flowers, seeds, orstems. Still further, the present invention provides cotton plantsregenerated from the tissue cultures of the invention.

In addition to the exemplary aspects and embodiments described above,further aspects and embodiments will become apparent by study of thefollowing descriptions.

DEFINITIONS

In the description and tables which follow, a number of terms are used.In order to provide a clear and consistent understanding of thespecification and claims, including the scope to be given such terms,the following definitions are provided:

100 seeds. As used herein “100 seeds” is a measurement in grams of thetotal weight of one hundred seeds.

Allele. Allele is any of one or more alternative forms of a gene, all ofwhich relate to one trait or characteristic. In a diploid cell ororganism, the two alleles of a given gene occupy corresponding loci on apair of homologous chromosomes.

Backcrossing. Backcrossing is a process in which a breeder repeatedlycrosses hybrid progeny back to one of the parents, for example, a firstgeneration hybrid F₁ with one of the parental genotypes of the F₁hybrid.

Kleistogamia or cleistogamy. As used herein ‘Kleistogamia orcleistogamy” is a trait of certain plants to propagate by usingnon-opening, self-pollinating flowers.

Disease Resistance. As used herein, the term “disease resistance” isdefined as the ability of plants to restrict the activities of aspecified pest, such as an insect, fungus, virus, or bacterial.

Disease Tolerance. As used herein, the term “disease tolerance” isdefined as the ability of plants to endure a specified pest (such as aninsect, fungus, virus or bacteria) or an adverse environmental conditionand still perform and produce in spite of this disorder.

Essentially all of the physiological and morphological characteristics.Essentially all of the physiological and morphological characteristicsmeans a plant having essentially all of the physiological andmorphological characteristics of the recurrent parent, except for thecharacteristics derived from the converted trait.

Elongation (E1). As used herein, the term “elongation” is defined as themeasure of elasticity of a bundle of fibers as measured by HVI.

Length (LEN). As used herein, the term “length” is defined as 2.5% spanlength in inches of fiber as measured by High Volume Instrumentation(HVI).

Fiber Strength (STR). As used herein, the term “fiber strength” isdefined as the force required to break a bundle of fibers as measured ingrams per millitex on the HVI.

Fruiting Nodes. As used herein, the term “fruiting nodes” is defined asthe number of nodes on the main stem from which arise branches whichbear fruit or bolls.

Gin Turnout (GTO). As used herein, the term “gin turnout” is defined asa fraction of lint in a machine harvested sample of seed cotton (lint,seed, and trash).

Lint/Boll. As used herein, the term “lint/boll” is the weight of lintper boll.

Lint Index. As used herein, the term “lint index” refers to the weightof lint per seed in milligrams.

Lint Percent. As used herein, the term “lint percent” is defined as thelint (fiber) fraction of seed cotton (lint and seed). Also known as lintturnout.

Lint Yield. As used herein, the term “lint yield” is defined as themeasure of the quantity of fiber produced on a given unit of land.Presented below in pounds of lint per acre.

Maturity. As used herein, the term “maturity” is defined as the HVImachine rating which refers to the degree of development of thickeningof the fiber cell wall relative to the perimeter or effective diameterof the fiber.

Maturity Rating (MAT). As used herein, the term “maturity rating” isdefined as a visual rating of plants of a variety, when 50% of allplants in two middle rows have at least one open boll.

Micronaire (MIC). As used herein, the term “micronaire” is defined as ameasure of the fineness of the fiber. Within a cotton cultivar,micronaire is also a measure of maturity. Micronaire differences aregoverned by changes in perimeter or in cell wall thickness, or bychanges in both. Within a cultivar, cotton perimeter is fairly constantand maturity will cause a change in micronaire. Consequently, micronairehas a high correlation with maturity within a variety of cotton.Maturity is the degree of development of cell wall thickness. Micronairemay not have a good correlation with maturity between varieties ofcotton having different fiber perimeter. Micronaire values range fromabout 2.0 to 6.0:

Below 2.9 Very fine Possible small perimeter but mature (good fiber), orlarge perimeter but immature (bad fiber). 2.9 to 3.7 Fine Variousdegrees of maturity and/or perimeter. 3.8 to 4.6 Average Average degreeof maturity and/or perimeter. 4.7 to 5.5 Coarse Usually fully-developed(mature), but larger perimeter. 5.6+ Very coarse Fully-developed,large-perimeter fiber.

Plant Height. As used herein, the term “plant height” is defined as theaverage height in inches or centimeters of a group of plants.

Seed/boll. As used herein, the term “seed/boll” refers to the number ofseeds per boll.

Weight of boll (BOLL). As used herein, the term “weight of boll” refersto the weight of a cotton boll in grams.

Single Trait Converted (Conversion). Single trait converted (conversion)plant refers to plants which are developed by a plant breeding techniquecalled backcrossing or via genetic engineering wherein essentially allof the desired morphological and physiological characteristics of avariety are recovered in addition to the single trait transferred intothe variety via the backcrossing technique or via genetic engineering.

Stremma (str). As used herein, the term “stremma” is defined as 1/10 ofa hectare.

Vegetative Nodes. As used herein, the term “vegetative nodes” is definedas the number of nodes from the cotyledonary node to the first fruitingbranch on the main stem of the plant.

DETAILED DESCRIPTION OF THE INVENTION

Cotton cultivar L-9009-6 is a Gossypium barbadense L. cotton varietywhich has shown uniformity and stability, as described in the followingVariety Description Information. It has been self-pollinated asufficient number of generations with careful attention to uniformity ofplant type. The cultivar has been increased with continued observationto uniformity.

Cotton cultivar L-9009-6 has the following morphologic and othercharacteristics from data taken in the Thiva region of Greece.

TABLE 1 VARIETY DESCRIPTION INFORMATION Species: Gossypium barbadense L.General: Plant Habit: Columnar Foliage: Medium Stem Lodging: ResistantFruiting Branch: Absent (Bolls emerge directly from the main stem)Growth: Indeterminate Leaf Color: Dark green Boll Shape: ConicalMaturity: Date of 50% open bolls: 105 days to 120 days Leaf (Upper-most,fully expanded leaf): Type: Palmate to digitate Pubescence: MediumNectaries: Present Glands: Leaf: Present Bract size: Medium Flower:Petals (color): Yellow Pollen (color): Yellow Petal Spot: Medium Seed:Seed Index: 11.3 Boll: Gin turnout: 37.2-38.8 Grams Seed Cotton perBoll: 3.6-3.96 Number of Locules per Boll: 3 to 4 Boll Type: Open FiberProperties: Method (HVI or other): HVI Length (inches, 2.5% SL):1.38-1.39 Uniformity: 87.9-88.8% Strength(g/tex): 38.8-39.7 Elongation:5.5-5.9% Micronaire: 4.2-4.3

The performance characteristics of cotton cultivar L-9009-6 were alsoanalyzed, as shown in Table 2, Table 3 and Table 4. In Table 2, Table 3,and Table 4, L-9009-6 was tested from four sites within in the Thivaregion of Greece with a sowing date of May 17, 2009 against cottoncultivars 856-1, L-403, L-1000 and 61029 as well as the commercialcotton cultivars, (Gossypium hirsutum) 9030, 9023 and 9099. Germinationdate was May 27, 2009, with a thinning date of Jun. 10, 2009 and apicking date of October 21, 2009.

Two tests were established based on a distance between rows of 49 cm and98 cm. The first test had four replications of L-9009-6 in the Thivaregion of Greece with a sowing date of May 17, 2009 compared with cottoncultivars 856-1, L-403, L-1000 and 61029 as well as the commercialcotton cultivars, (Gossypium hirsutum) 9030, 9023 and 9099 on randomlylocated sites with a distance between rows of 49 cm with one plot offour rows per 10 meters each. The distance between each plant was 10 cm.Sowing was by manual sowing machine and picking two middle rows by hand.Irrigation was by irrigation boom and the sites were irrigated on May22, 2009, Jun. 26, 2009, Jul. 12, 2009, Jul. 27, 2009, Aug. 7, 2009 andSep. 18, 2009. A N15, P15, and K15 type fertilizer was applied on May17, 2009 at a rate of 30 kg/str. A urea type fertilizer was applied onJun. 19, 2009 at a rate of 30 kg/str and a third urea type fertilizerwas applied on Jul. 11, 2009 at a rate of 20 kg/str. A cotorane typepesticide was applied on May 17, 2009.

The second test type L-9009-6 was tested from four sites within in theThiva region of Greece with a sowing date of May 17, 2009 against cottoncultivars L-403, L-1000, 856-1, and 61029 as well as the commercialcotton cultivars, (Gossypium hirsutum) 9030, 9023 and 9099 on randomlylocated sites with a distance between rows of 98 cm. One plot of fourrows per 10 meters each. The distance between each plant was 10 cm.Sowing was by manual sowing machine and picking two middle rows by hand.Irrigation was by irrigation boom and the sites were irrigated on May22, 2009, Jun. 26, 2009, Jul. 12, 2009, Jul. 27, 2009, Aug. 7, 2009 andSep. 18, 2009. A N15, P15, and K15 type fertilizer was applied on May17, 2009 at a rate of 30 kg/str. A urea type fertilizer was applied onJun. 19, 2009 at a rate of 30 kg/str and a third urea type fertilizerwas applied on Jul. 11, 2009 at a rate of 20 kg/str. A cotorane typepesticide was applied on May 17, 2009.

Sampling began one to two days before first picking (when approximately65% to 80% of the bolls are open, with a second picking from eachvariety and each of the four replications). The two middle rows of eachreplication were chosen and 100 bolls were randomly selected. Onlymature open bolls were selected. Pick was by hand and each variety wasweighed separately.

In Table 2, the first column shows the variety name. Column two showstotal yield in (kg/ha) for four sites with a distance between rows of 98cm. Column three shows the total yield in (kg/ha) for four sites with adistance between rows of 49 cm. Column four shows the percent differencebetween the total yield for plants shown in rows with a distance of 98cm and a distance of 49 cm. Column five shows total lint yield in(kg/ha) for four sites with a distance between rows of 98 cm. Column sixshows the total lint yield in (kg/ha) for four sites with a distancebetween rows of 49 cm. Column seven shows the percent difference betweenthe total lint yield for plants shown in rows with a distance of 98 cmand a distance of 49 cm. Column eight shows maturity in days for foursites with a distance between rows of 98 cm. Column nine shows thematurity in days for four sites with a distance between rows of 49 cm.Column ten shows the percent difference between the maturity in days forplants shown in rows with a distance of 98 cm and a distance of 49 cm.

TABLE 2 Total Total Total lint Total lint yield yield yield yield MATMAT kg/ha kg/ha kg/ha kg/ha (days) (days) VARIETY 98 cm 49 cm DIFF. % 98cm 49 cm DIFF. % 98 cm 49 cm DIFF. % L-403 2782 4213 151 977 1483 152118.5 115.5 97 L-1000 2962 4127 139 1035 1460 141 118.0 116.0 98 856-12667 3482 131 1118 1516 136 118.5 114.5 97 L-9009-6 3020 3851 128 11231422 127 119.5 115.5 97 61029 3104 3778 122 1052 1344 128 117.5 114.5 97 9030 4990 5260 105 1752 1869 107 107.0 107.5 100  9023 4298 4483 1041593 1612 101 110.0 107.5 98  9099 4249 4376 103 1702 1699 100 110.0110.8 101 AVERAGE 3510 4200 123 1290 1550 124 115 113 98

In Table 3, the first column shows the variety name. Column two showsthe fiber length (LEN) in millimeters for four sites with a distancebetween rows of 98 cm. Column three shows the fiber length inmillimeters for four sites with a distance between rows of 49 cm. Columnfour shows the percent difference between the fiber length for plantsgrown in rows with a distance of 98 cm and a distance of 49 cm. Columnfive shows fiber strength (STR) for four sites with a distance betweenrows of 98 cm. Column six shows the fiber strength for four sites with adistance between rows of 49 cm. Column seven shows the percentdifference between the fiber strength for plants grown in rows with adistance of 98 cm and a distance of 49 cm. Column eight shows micronaire(MIC) for four sites with a distance between rows of 98 cm. Column nineshows the micronaire for four sites with a distance between rows of 49cm. Column ten shows the percent difference between the micronaire forplants grown in rows with a distance of 98 cm and a distance of 49 cm.

TABLE 3 LEN LEN (mm) (mm) STR STR MIC MIC VARIETY 98 cm 49 cm DIFF. % 98cm 49 cm DIFF. % 98 cm 49 cm DIFF. % L-403 34.7 34.9 101 37.8 37.5 994.6 4.4 94 L-1000 34.7 35.0 101 39.4 37.8 96 4.5 4.3 95 856-1 33.1 32.598 36.6 36.7 100 4.3 4.2 97 L-9009-6 34.1 34.5 101 34.7 36.7 106 4.3 4.196 61029 35.3 35.4 100 38.0 36.4 96 4.2 3.9 92  9030 30.6 30.1 98 31.931.7 99 3.8 3.8 101  9023 29.9 29.5 98 31.8 31.0 97 4.1 4.1 99  909929.7 29.8 100 31.3 30.9 99 4.0 3.8 96 AVERAGE 33 33 100 35 35 99 4 4 96

In Table 4, the first column shows the variety name. Column two showsthe gin turn out (GTO) in millimeters for four sites with a distancebetween rows of 98 cm. Column three shows the gin turn out inmillimeters for four sites with a distance between rows of 49 cm. Columnfour shows the percent difference between the gin turn out for plantsgrown in rows with a distance of 98 cm and a distance of 49 cm. Columnfive shows the weight of a boll in grams (BOLL) for four sites with adistance between rows of 98 cm. Column six shows the weight of a bollfor four sites with a distance between rows of 49 cm. Column seven showsthe difference between the weight of a boll for plants grown in rowswith a distance of 98 cm and a distance of 49 cm. Column eight showsweight of 100 seeds in grams for four sites with a distance between rowsof 98 cm. Column nine shows the weight of 100 seeds in grams for foursites with a distance between rows of 49 cm. Column ten shows thepercent difference between the weight of 100 seeds in grams for plantsgrown in rows with a distance of 98 cm and a distance of 49 cm.

TABLE 4 100 100 GTO GTO BOLL BOLL SEEDS SEEDS (mm) (mm) (g) (g) (g) (g)VARIETY 98 cm 49 cm DIFF. % 98 cm 49 cm DIFF. % 98 cm 49 cm DIFF. %L-403 35.1 35.2 100 4.0 3.5 87 13.5 12.7 94 L-1000 34.9 35.4 101 3.9 3.691 13.3 12.8 96 856-1 41.9 43.6 104 3.8 3.3 85 10.9 9.8 91 L-9009-6 37.236.9 99 4.1 3.6 88 11.2 10.3 92 61029 33.9 35.6 105 4.3 3.7 85 13.0 11.488  9030 35.1 35.5 101 6.2 5.9 95 10.9 11.0 102  9023 37.1 36.0 97 5.95.3 91 11.6 11.6 100  9099 40.0 38.8 97 5.9 5.5 93 10.5 10.7 102 AVERAGE37 37 101 5 4 89 12 11 95

This invention is also directed to methods for producing a cotton plantby crossing a first parent cotton plant with a second parent cottonplant, wherein the first or second cotton plant is the cotton plant fromthe cultivar L-9009-6. Further, both the first and second parent cottonplants may be the cultivar L-9009-6 (e.g., self-pollination). Therefore,any methods using the cultivar L-9009-6 are part of this invention:selling, backcrosses, hybrid breeding, and crosses to populations. Anyplants produced using cultivar L-9009-6 as parents are within the scopeof this invention. As used herein, the term “plant” includes plantcells, plant protoplasts, plant cells of tissue culture from whichcotton plants can be regenerated, plant calli, plant clumps, and plantcells that are intact in plants or parts of plants, such as pollen,flowers, embryos, ovules, seeds, leaves, stems, roots, anthers, pistils,and the like. Thus, another aspect of this invention is to provide forcells which upon growth and differentiation produce a cultivar havingessentially all of the physiological and morphological characteristicsof L-9009-6.

The present invention contemplates a cotton plant regenerated from atissue culture of a cultivar (e.g., L-9009-6) or hybrid plant of thepresent invention. As is well known in the art, tissue culture of cottoncan be used for the in vitro regeneration of a cotton plant. Tissueculture of various tissues of cotton and regeneration of plants therefrom is well known and widely published.

Further Embodiments of the Invention

With the advent of molecular biological techniques that have allowed theisolation and characterization of genes that encode specific proteinproducts, scientists in the field of plant biology developed a stronginterest in engineering the genome of plants to contain and expressforeign genes, or additional, or modified versions of native, orendogenous, genes (perhaps driven by different promoters) in order toalter the traits of a plant in a specific manner. Such foreignadditional and/or modified genes are referred to herein collectively as“transgenes.” Over the last fifteen to twenty years several methods forproducing transgenic plants have been developed, and the presentinvention, in particular embodiments, also relates to transformedversions of the claimed cultivar.

Plant transformation involves the construction of an expression vectorwhich will function in plant cells. Such a vector comprises DNAcomprising a gene under control of or operatively linked to a regulatoryelement (for example, a promoter). The expression vector may contain oneor more such operably linked gene/regulatory element combinations. Thevector(s) may be in the form of a plasmid, and can be used alone or incombination with other plasmids, to provide transformed cotton plants,using transformation methods as described below to incorporatetransgenes into the genetic material of the cotton plant(s).

Expression Vectors for Cotton Transformation: Marker Genes

Expression vectors include at least one genetic marker operably linkedto a regulatory element (for example, a promoter) that allowstransformed cells containing the marker to be either recovered bynegative selection (i e , inhibiting growth of cells that do not containthe selectable marker gene), or by positive selection (i.e., screeningfor the product encoded by the genetic marker). Many commonly usedselectable marker genes for plant transformation are well known in thetransformation arts, and include, for example, genes that code forenzymes that metabolically detoxify a selective chemical agent which maybe an antibiotic or an herbicide, or genes that encode an altered targetwhich is insensitive to the inhibitor. A few positive selection methodsare also known in the art.

One commonly used selectable marker gene for plant transformation is theneomycin phosphotransferase II (nptII), which, when under the control ofplant regulatory signals, confers resistance to kanamycin. Fraley, etal., PNAS, 80:4803 (1983). Another commonly used selectable marker geneis the hygromycin phosphotransferase gene which confers resistance tothe antibiotic hygromycin. Vanden Elzen, et al., Plant Mol. Biol., 5:299(1985).

Additional selectable marker genes of bacterial origin that conferresistance to antibiotics include gentamycin acetyl transferase,streptomycin phosphotransferase, and aminoglycoside-3′-adenyltransferase, the bleomycin resistance determinant. Hayford, et al.,Plant Physiol., 86:1216 (1988); Jones, et al., Mol. Gen. Genet., 210:86(1987); Svab, et al., Plant Mol. Biol., 14:197 (1990); Hille, et al.,Plant Mol. Biol., 7:171 (1986). Other selectable marker genes conferresistance to herbicides such as glyphosate, glufosinate, or bromoxynil.Comai, et al., Nature, 317:741-744 (1985); Gordon-Kamm, et al., PlantCell, 2:603-618 (1990); and Stalker, et al., Science, 242:419-423(1988).

Other selectable marker genes for plant transformation that are not ofbacterial origin include, for example, mouse dihydrofolate reductase,plant 5-enolpyruvyl-shikimate-3-phosphate synthase and plantacetolactate synthase. Eichholtz, et al., Somatic Cell Mol. Genet.,13:67 (1987); Shah, et al., Science, 233:478 (1986); Charest, et al.,Plant Cell Rep., 8:643 (1990).

Another class of marker genes for plant transformation requiresscreening of presumptively transformed plant cells rather than directgenetic selection of transformed cells for resistance to a toxicsubstance such as an antibiotic. These genes are particularly useful toquantify or visualize the spatial pattern of expression of a gene inspecific tissues and are frequently referred to as reporter genesbecause they can be fused to a gene or gene regulatory sequence for theinvestigation of gene expression. Commonly used genes for screeningpresumptively transformed cells include β-glucuronidase (GUS),β-galactosidase, luciferase, and chloramphenicol acetyltransferase.Jefferson, R. A., Plant Mol. Biol. Rep., 5:387 (1987); Teeri, et al.,EMBO J., 8:343 (1989); Koncz, et al., PNAS, 84:131 (1987); DeBlock, etal., EMBO J. 3:1681 (1984).

In vivo methods for visualizing GUS activity that do not requiredestruction of plant tissue are available. Molecular Probes Publication2908, IMAGENE GREEN, pp. 1-4 (1993) and Naleway, et al., J. Cell Biol.,115:151a (1991). However, these in vivo methods for visualizing GUSactivity have not proven useful for recovery of transformed cellsbecause of low sensitivity, high fluorescent backgrounds, andlimitations associated with the use of luciferase genes as selectablemarkers.

More recently, a gene encoding Green Fluorescent Protein (GFP) has beenutilized as a marker for gene expression in prokaryotic and eukaryoticcells. Chalfie, et al., Science, 263:802 (1994). GFP and mutants of GFPmay be used as screenable markers

Expression Vectors for Cotton Transformation: Promoters

Genes included in expression vectors must be driven by a nucleotidesequence comprising a regulatory element (for example, a promoter).Several types of promoters are now well known in the transformationarts, as are other regulatory elements that can be used alone or incombination with promoters.

As used herein, “promoter” includes reference to a region of DNAupstream from the start of transcription and involved in recognition andbinding of RNA polymerase and other proteins to initiate transcription.A “plant promoter” is a promoter capable of initiating transcription inplant cells. Examples of promoters under developmental control includepromoters that preferentially initiate transcription in certain tissues,such as leaves, roots, seeds, fibers, xylem vessels, tracheids, orsclerenchyma. Such promoters are referred to as “tissue-preferred.”Promoters which initiate transcription only in certain tissue arereferred to as “tissue-specific.” A “cell type” specific promoterprimarily drives expression in certain cell types in one or more organs,for example, vascular cells in roots or leaves. An “inducible” promoteris a promoter which is under environmental control. Examples ofenvironmental conditions that may effect transcription by induciblepromoters include anaerobic conditions or the presence of light.Tissue-specific, tissue-preferred, cell type specific, and induciblepromoters constitute the class of “non-constitutive” promoters. A“constitutive” promoter is a promoter which is active under mostenvironmental conditions.

A. Inducible Promoters:

An inducible promoter is operably linked to a gene for expression incotton. Optionally, the inducible promoter is operably linked to anucleotide sequence encoding a signal sequence which is operably linkedto a gene for expression in cotton. With an inducible promoter the rateof transcription increases in response to an inducing agent.

Any inducible promoter can be used in the instant invention. See Ward,et al., Plant Mol. Biol., 22:361-366 (1993). Exemplary induciblepromoters include, but are not limited to, that from the ACEI systemwhich responds to copper (Mett, et al., PNAS, 90:4567-4571 (1993)); In2gene from maize which responds to benzenesulfonamide herbicide safeners(Hershey, et al., Mol. Gen. Genet., 227:229-237 (1991) and Gatz, et al.,Mol. Gen. Genet., 243:32-38 (1994)); or Tet repressor from Tn10 (Gatz,et al., Mol. Gen. Genet., 227:229-237 (1991)). A particularly preferredinducible promoter is a promoter that responds to an inducing agent towhich plants do not normally respond. An exemplary inducible promoter isthe inducible promoter from a steroid hormone gene, the transcriptionalactivity of which is induced by a glucocorticosteroid hormone (Schena,et al., PNAS, 88:0421 (1991)).

B. Constitutive Promoters:

A constitutive promoter is operably linked to a gene for expression incotton or the constitutive promoter is operably linked to a nucleotidesequence encoding a signal sequence which is operably linked to a genefor expression in cotton.

Many different constitutive promoters can be utilized in the instantinvention. Exemplary constitutive promoters include, but are not limitedto, the promoters from plant viruses such as the 35S promoter from CaMV(Odell, et al., Nature, 313:810-812 (1985)) and the promoters from suchgenes as rice actin (McElroy, et al., Plant Cell, 2:163-171 (1990));ubiquitin (Christensen, et al., Plant Mol. Biol., 12:619-632 (1989) andChristensen, et al., Plant Mol. Biol., 18:675-689 (1992)); pEMU (Last,et al., Theor. Appl. Genet., 81:581-588 (1991)); MAS (Velten, et al.,EMBO J., 3:2723-2730 (1984)); and maize H3 histone (Lepetit, et al.,Mol. Gen. Genet., 231:276-285 (1992) and Atanassova, et al., PlantJournal, 2 (3): 291-300 (1992)).

The ALS promoter, Xbal/Ncol fragment 5′ to the Brassica napus ALS3structural gene (or a nucleotide sequence similarity to said Xbal/Ncolfragment), represents a particularly useful constitutive promoter. SeePCT Application No. WO 96/30530.

C. Tissue-Specific or Tissue-Preferred Promoters:

A tissue-specific promoter is operably linked to a gene for expressionin cotton. Optionally, the tissue-specific promoter is operably linkedto a nucleotide sequence encoding a signal sequence which is operablylinked to a gene for expression in cotton. Plants transformed with agene of interest operably linked to a tissue-specific promoter producethe protein product of the transgene exclusively, or preferentially, ina specific tissue.

Any tissue-specific or tissue-preferred promoter can be utilized in theinstant invention. Exemplary tissue-specific or tissue-preferredpromoters include, but are not limited to, a root-preferred promoter,such as that from the phaseolin gene (Murai, et al., Science, 23:476-482(1983) and Sengupta-Gopalan, et al., PNAS, 82:3320-3324 (1985)); aleaf-specific and light-induced promoter, such as that from cab orrubisco (Simpson, et al., EMBO J., 4(11):2723-2729 (1985) and Timko, etal., Nature, 318:579-582 (1985)); an anther-specific promoter, such asthat from LAT52 (Twell, et al., Mol. Gen. Genet., 217:240-245 (1989)); apollen-specific promoter, such as that from Zm13 (Guerrero, et al., Mol.Gen. Genet., 244:161-168 (1993)); or a microspore-preferred promoter,such as that from apg (Twell, et al., Sex. Plant Reprod., 6:217-224(1993)).

Signal Sequences for Targeting Proteins to Subcellular Compartments

Transport of protein produced by transgenes to a subcellularcompartment, such as the chloroplast, vacuole, peroxisome, glyoxysome,cell wall, or mitochondrion, or for secretion into the apoplast, isaccomplished by means of operably linking the nucleotide sequenceencoding a signal sequence to the 5′ and/or 3′ region of a gene encodingthe protein of interest. Targeting sequences at the 5′ and/or 3′ end ofthe structural gene may determine, during protein synthesis andprocessing, where the encoded protein is ultimately compartmentalized.

The presence of a signal sequence directs a polypeptide to either anintracellular organelle or subcellular compartment or for secretion tothe apoplast. Many signal sequences are known in the art. See, forexample, Becker, et al., Plant Mol. Biol., 20:49 (1992); Close, P. S.,Master's Thesis, Iowa State University (1993); Knox, C., et al., PlantMol. Biol., 9:3-17 (1987); Lerner, et al., Plant Physiol., 91:124-129(1989); Fontes, et al., Plant Cell, 3:483-496 (1991); Matsuoka, et al.,PNAS, 88:834 (1991); Gould, et al., J. Cell. Biol., 108:1657 (1989);Creissen, et al., Plant J., 2:129 (1991); Kalderon, et al., Cell,39:499-509 (1984); Steifel, et al., Plant Cell, 2:785-793 (1990).

Foreign Protein Genes and Agronomic Genes

With transgenic plants according to the present invention, a foreignprotein can be produced in commercial quantities. Thus, techniques forthe selection and propagation of transformed plants, which are wellunderstood in the art, yield a plurality of transgenic plants which areharvested in a conventional manner, and a foreign protein then can beextracted from a tissue of interest or from total biomass. Proteinextraction from plant biomass can be accomplished by known methods whichare discussed, for example, by Heney and Orr, Anal. Biochem., 114:92-6(1981).

According to a preferred embodiment, the transgenic plant provided forcommercial production of foreign protein is a cotton plant. In anotherpreferred embodiment, the biomass of interest is seed. For therelatively small number of transgenic plants that show higher levels ofexpression, a genetic map can be generated, primarily via conventionalRFLP, PCR, and SSR analysis, which identifies the approximatechromosomal location of the integrated DNA molecule. For exemplarymethodologies in this regard, see Glick and Thompson, Methods in PlantMolecular Biology and Biotechnology, CRC Press, Boca Raton, 269:284(1993). Map information concerning chromosomal location is useful forproprietary protection of a subject transgenic plant. If unauthorizedpropagation is undertaken and crosses made with other germplasm, the mapof the integration region can be compared to similar maps for suspectplants, to determine if the latter have a common parentage with thesubject plant. Map comparisons would involve hybridizations, RFLP, PCR,SSR and sequencing, all of which are conventional techniques.

Likewise, by means of the present invention, agronomic genes can beexpressed in transformed plants. More particularly, plants can begenetically engineered to express various phenotypes of agronomicinterest. Exemplary genes implicated in this regard include, but are notlimited to, those categorized below:

A. Genes that Confer Resistance to Pests or Disease and that Encode:

1. Plant disease resistance genes. Plant defenses are often activated byspecific interaction between the product of a disease resistance gene(R) in the plant and the product of a corresponding avirulence (Avr)gene in the pathogen. A plant variety can be transformed with clonedresistance gene to engineer plants that are resistant to specificpathogen strains. See, for example, Jones, et al., Science, 266:789(1994) (cloning of the tomato Cf-9 gene for resistance to Cladosporiumfulvum); Martin, et al., Science, 262:1432 (1993) (tomato Pto gene forresistance to Pseudomonas syringae pv. tomato encodes a protein kinase);Mindrinos, et al., Cell, 78:1089 (1994) (Arabidopsis RSP2 gene forresistance to Pseudomonas syringae).

2. A gene conferring resistance to a pest, such as nematodes. See, e.g.,PCT Application No. WO 96/30517; PCT Application No. WO 93/19181.

3. A Bacillus thuringiensis protein, a derivative thereof or a syntheticpolypeptide modeled thereon. See, for example, Geiser, et al., Gene,48:109 (1986), who disclose the cloning and nucleotide sequence of a Btδ-endotoxin gene. Moreover, DNA molecules encoding δ-endotoxin genes canbe purchased from American Type Culture Collection, Manassas, Va., forexample, under ATCC Accession Nos. 40098, 67136, 31995, and 31998.

4. A lectin. See, for example, the disclosure by Van Damme, et al.,Plant Molec. Biol., 24:25 (1994), who disclose the nucleotide sequencesof several Clivia miniata mannose-binding lectin genes.

5. A vitamin-binding protein such as avidin. See PCT Application No. US93/06487. The application teaches the use of avidin and avidinhomologues as larvicides against insect pests.

6. An enzyme inhibitor, for example, a protease or proteinase inhibitoror an amylase inhibitor. See, for example, Abe, et al., J. Biol. Chem.,262:16793 (1987) (nucleotide sequence of rice cysteine proteinaseinhibitor); Huub, et al., Plant Molec. Biol., 21:985 (1993) (nucleotidesequence of cDNA encoding tobacco proteinase inhibitor I); Sumitani, etal., Biosci. Biotech. Biochem., 57:1243 (1993) (nucleotide sequence ofStreptomyces nitrosporeus α-amylase inhibitor); and U.S. Pat. No.5,494,813 (Hepher and Atkinson, issued Feb. 27, 1996).

7. An insect-specific hormone or pheromone, such as an ecdysteroid andjuvenile hormone, a variant thereof, a mimetic based thereon, or anantagonist or agonist thereof. See, for example, the disclosure byHammock, et al., Nature, 344:458 (1990), of baculovirus expression ofcloned juvenile hormone esterase, an inactivator of juvenile hormone.

8. An insect-specific peptide or neuropeptide which, upon expression,disrupts the physiology of the affected pest. For example, see thedisclosures of Regan, J. Biol. Chem., 269:9 (1994) (expression cloningyields DNA coding for insect diuretic hormone receptor) and Pratt, etal., Biochem. Biophys. Res. Comm., 163:1243 (1989) (an allostatin isidentified in Diploptera puntata). See also, U.S. Pat. No. 5,266,317 toTomalski, et al., who disclose genes encoding insect-specific, paralyticneurotoxins.

9. An insect-specific venom produced in nature by a snake, a wasp, etc.For example, see Pang, et al., Gene, 116:165 (1992), for disclosure ofheterologous expression in plants of a gene coding for a scorpioninsectotoxic peptide.

10. An enzyme responsible for a hyper-accumulation of a monoterpene, asesquiterpene, a steroid, a hydroxamic acid, a phenylpropanoidderivative, or another non-protein molecule with insecticidal activity.

11. An enzyme involved in the modification, including thepost-translational modification, of a biologically active molecule. Forexample, a glycolytic enzyme, a proteolytic enzyme, a lipolytic enzyme,a nuclease, a cyclase, a transaminase, an esterase, a hydrolase, aphosphatase, a kinase, a phosphorylase, a polymerase, an elastase, achitinase, and a glucanase, whether natural or synthetic. See PCTApplication No. WO 93/02197 in the name of Scott, et al., whichdiscloses the nucleotide sequence of a callase gene. DNA molecules whichcontain chitinase-encoding sequences can be obtained, for example, fromthe ATCC under Accession Nos. 39637 and 67152. See also, Kramer, et al.,Insect Biochem. Molec. Biol., 23:691 (1993), who teach the nucleotidesequence of a cDNA encoding tobacco hornworm chitinase, and Kawalleck,et al., Plant Molec. Biol., 21:673 (1993), who provide the nucleotidesequence of the parsley ubi4-2 polyubiquitin gene.

12. A molecule that stimulates signal transduction. For example, see thedisclosure by Botella, et al., Plant Molec. Biol., 24:757 (1994), ofnucleotide sequences for mung bean calmodulin cDNA clones and Griess, etal., Plant Physiol., 104:1467 (1994), who provide the nucleotidesequence of a maize calmodulin cDNA clone.

13. A hydrophobic moment peptide. See PCT Application No. WO 95/16776(disclosure of peptide derivatives of Tachyplesin which inhibit fungalplant pathogens) and PCT Application No. WO 95/18855 (teaches syntheticantimicrobial peptides that confer disease resistance).

14. A membrane permease, a channel former or a channel blocker. Forexample, see the disclosure of Jaynes, et al., Plant Sci., 89:43 (1993),of heterologous expression of a cecropin-β lytic peptide analog torender transgenic tobacco plants resistant to Pseudomonas solanacearum.

15. A viral-invasive protein or a complex toxin derived therefrom. Forexample, the accumulation of viral coat proteins in transformed plantcells imparts resistance to viral infection and/or disease developmenteffected by the virus from which the coat protein gene is derived, aswell as by related viruses. See, Beachy, et al., Ann. Rev. Phytopathol.,28:451 (1990). Coat protein-mediated resistance has been conferred upontransformed plants against alfalfa mosaic virus, cucumber mosaic virus,tobacco streak virus, potato virus X, potato virus Y, tobacco etchvirus, tobacco rattle virus and tobacco mosaic virus. Id.

16. An insect-specific antibody or an immunotoxin derived therefrom.Thus, an antibody targeted to a critical metabolic function in theinsect gut would inactivate an affected enzyme, killing the insect. See,Taylor, et al., Abstract #497, Seventh Intl Symposium on MolecularPlant-Microbe Interactions (Edinburgh, Scotland) (1994) (enzymaticinactivation in transgenic tobacco via production of single-chainantibody fragments).

17. A virus-specific antibody. See, for example, Tavladoraki, et al.,Nature, 366:469 (1993), who show that transgenic plants expressingrecombinant antibody genes are protected from virus attack.

18. A developmental-arrestive protein produced in nature by a pathogenor a parasite. Thus, fungal endo-α-1,4-D-polygalacturonases facilitatefungal colonization and plant nutrient release by solubilizing plantcell wall homo-α-1,4-D-galacturonase. See, Lamb, et al., Bio/technology,10:1436 (1992). The cloning and characterization of a gene which encodesa bean endopolygalacturonase-inhibiting protein is described by Toubart,et al., Plant J., 2:367 (1992).

19. A developmental-arrestive protein produced in nature by a plant. Forexample, Logemann, et al., Bio/technology, 10:305 (1992), have shownthat transgenic plants expressing the barley ribosome-inactivating genehave an increased resistance to fungal disease.

B. Genes that Confer Resistance to an Herbicide:

1. An herbicide that inhibits the growing point or meristem, such as animidazolinone or a sulfonylurea. Exemplary genes in this category codefor mutant ALS and AHAS enzyme as described, for example, by Lee, etal., EMBO J., 7:1241 (1988), and Miki, et al., Theor. Appl. Genet.,80:449 (1990), respectively.

2. Glyphosate (resistance conferred by mutant5-enolpyruvlshikimate-3-phosphate synthase (EPSPS) and aroA genes,respectively) and other phosphono compounds such as glufosinate(phosphinothricin acetyl transferase (PAT) and Streptomyceshygroscopicus PAT bar genes), and pyridinoxy or phenoxy proprionic acidsand cyclohexones (ACCase inhibitor-encoding genes). See, for example,U.S. Pat. No. 4,940,835 to Shah, et al., which discloses the nucleotidesequence of a form of EPSP which can confer glyphosate resistance. A DNAmolecule encoding a mutant aroA gene can be obtained under ATCCAccession No. 39256, and the nucleotide sequence of the mutant gene isdisclosed in U.S. Pat. No. 4,769,061 to Comai. European PatentApplication No. 0 333 033 to Kumada, et al., and U.S. Pat. No. 4,975,374to Goodman, et al., disclose nucleotide sequences of glutaminesynthetase genes which confer resistance to herbicides such asL-phosphinothricin. The nucleotide sequence of a PAT gene is provided inEuropean Application No. 0 242 246 to Leemans, et al. DeGreef, et al.,Bio/technology, 7:61 (1989), describe the production of transgenicplants that express chimeric bar genes coding for PAT activity.Exemplary of genes conferring resistance to phenoxy proprionic acids andcyclohexones, such as sethoxydim and haloxyfop are the Acc1-S1, Acc1-S2,and Acc1-S3 genes described by Marshall, et al., Theor. Appl. Genet.,83:435 (1992).

3. An herbicide that inhibits photosynthesis, such as a triazine (psbAand gs+ genes) or a benzonitrile (nitrilase gene). Przibila, et al.,Plant Cell, 3:169 (1991), describe the transformation of Chlamydomonaswith plasmids encoding mutant psbA genes. Nucleotide sequences fornitrilase genes are disclosed in U.S. Pat. No. 4,810,648 to Stalker, andDNA molecules containing these genes are available under ATCC AccessionNos. 53435, 67441, and 67442. Cloning and expression of DNA coding for aglutathione S-transferase is described by Hayes, et al., Biochem. J.,285:173 (1992).

C. Genes that Confer or Contribute to a Value-Added Trait, Such as:

1. Modified fatty acid metabolism, for example, by transforming a plantwith an antisense gene of stearyl-ACP desaturase to increase stearicacid content of the plant. See Knultzon, et al., PNAS, 89:2624 (1992).

2. Decreased phytate content: (a) Introduction of a phytase-encodinggene would enhance breakdown of phytate, adding more free phosphate tothe transformed plant. See, for example, Van Hartingsveldt, et al.,Gene, 127:87 (1993), for a disclosure of the nucleotide sequence of anAspergillus niger phytase gene; and (b) A gene could be introduced thatreduced phytate content. For example, in maize, this could beaccomplished by cloning and then reintroducing DNA associated with thesingle allele which is responsible for maize mutants characterized bylow levels of phytic acid. See, Raboy, et al., Maydica, 35:383 (1990).

3. Modified carbohydrate composition effected, for example, bytransforming plants with a gene coding for an enzyme that alters thebranching pattern of starch. See, Shiroza, et al., J. Bacteol., 170:810(1988) (nucleotide sequence of Streptococcus mutantsfructosyltransferase gene); Steinmetz, et al., Mol. Gen. Genet., 20:220(1985) (nucleotide sequence of Bacillus subtilis levansucrase gene);Pen, et al., Bio/technology, 10:292 (1992) (production of transgenicplants that express Bacillus licheniformis α-amylase); Elliot, et al.,Plant Molec. Biol., 21:515 (1993) (nucleotide sequences of tomatoinvertase genes); Sørgaard, et al., J. Biol. Chem., 268:22480 (1993)(site-directed mutagenesis of barley α-amylase gene); and Fisher, etal., Plant Physiol., 102:1045 (1993) (maize endosperm starch branchingenzyme II).

Methods for Cotton Transformation

Numerous methods for plant transformation have been developed, includingbiological and physical, plant transformation protocols. See, forexample, Mild, et al., “Procedures for Introducing Foreign DNA intoPlants” in Methods in Plant Molecular Biology and Biotechnology, Glickand Thompson (Eds.), CRC Press, Inc., Boca Raton, pp. 67-88 (1993). Inaddition, expression vectors and in vitro culture methods for plant cellor tissue transformation and regeneration of plants are available. See,for example, Gruber, et al., “Vectors for Plant Transformation” inMethods in Plant Molecular Biology and Biotechnology, Glick and Thompson(Eds.), CRC Press, Inc., Boca Raton, pp. 89-119 (1993).

A. Agrobacterium-Mediated Transformation:

One method for introducing an expression vector into plants is based onthe natural transformation system of Agrobacterium. See, for example,Horsch, et al., Science, 227:1229 (1985). A. tumefaciens and A.rhizogenes are plant pathogenic soil bacteria which geneticallytransform plant cells. The Ti and Ri plasmids of A. tumefaciens and A.rhizogenes, respectively, carry genes responsible for genetictransformation of the plant. See, for example, Kado, C. I., Crit. Rev.Plant Sci., 10:1 (1991). Descriptions of Agrobacterium vector systemsand methods for Agrobacterium-mediated gene transfer are provided byGruber, et al., supra, Miki, et al., supra, and Moloney, et al., PlantCell Rep., 8:238 (1989). See also, U.S. Pat. No. 5,563,055 (Townsend andThomas), issued Oct. 8, 1996.

B. Direct Gene Transfer:

Several methods of plant transformation, collectively referred to asdirect gene transfer, have been developed as an alternative toAgrobacterium-mediated transformation. A generally applicable method ofplant transformation is microprojectile-mediated transformation whereinDNA is carried on the surface of microprojectiles measuring 1 μm to 4μm. The expression vector is introduced into plant tissues with abiolistic device that accelerates the microprojectiles to speeds of 300m/s to 600 m/s which is sufficient to penetrate plant cell walls andmembranes. Sanford, et al., Part. Sci. Technol., 5:27 (1987); Sanford,J. C., Trends Biotech., 6:299 (1988); Klein, et al., Bio/technology,6:559-563 (1988); Sanford, J. C., Physiol Plant, 7:206 (1990); Klein, etal., Bio/technology, 10:268 (1992). See also, U.S. Pat. No. 5,015,580(Christou, et al.), issued May 14, 1991; U.S. Pat. No. 5,322,783 (Tomes,et al.), issued Jun. 21, 1994.

Another method for physical delivery of DNA to plants is sonication oftarget cells. Zhang, et al., Bio/technology, 9:996 (1991).Alternatively, liposome and spheroplast fusion have been used tointroduce expression vectors into plants. Deshayes, et al., EMBO J.,4:2731 (1985); Christou, et al., PNAS, 84:3962 (1987). Direct uptake ofDNA into protoplasts using CaCl₂ precipitation, polyvinyl alcohol, orpoly-L-ornithine has also been reported. Hain, et al., Mol. Gen. Genet.,199:161 (1985) and Draper, et al., Plant Cell Physiol. 23:451 (1982).Electroporation of protoplasts and whole cells and tissues has also beendescribed. Donn, et al., In Abstracts of VIIth International Congress onPlant Cell and Tissue Culture IAPTC, A2-38, p. 53 (1990); D'Halluin, etal., Plant Cell, 4:1495-1505 (1992); and Spencer, et al., Plant Mol.Biol., 24:51-61 (1994).

Following transformation of cotton target tissues, expression of theabove-described selectable marker genes allows for preferentialselection of transformed cells, tissues, and/or plants, usingregeneration and selection methods now well known in the art.

The foregoing methods for transformation would typically be used forproducing a transgenic variety. The transgenic variety could then becrossed, with another (non-transformed or transformed) variety, in orderto produce a new transgenic variety. Alternatively, a genetic traitwhich has been engineered into a particular cotton cultivar using theforegoing transformation techniques could be moved into another cultivarusing traditional backcrossing techniques that are well known in theplant breeding arts. For example, a backcrossing approach could be usedto move an engineered trait from a public, non-elite variety into anelite variety, or from a variety containing a foreign gene in its genomeinto a variety or varieties which do not contain that gene. As usedherein, “crossing” can refer to a simple X by Y cross, or the process ofbackcrossing, depending on the context.

Single-Gene Conversion

When the term “cotton plant” is used in the context of the presentinvention, this also includes any single gene conversions of thatvariety. The term “single gene converted plant” as used herein refers tothose cotton plants which are developed by a plant breeding techniquecalled backcrossing wherein essentially all of the desired morphologicaland physiological characteristics of a variety are recovered in additionto the single gene transferred into the variety via the backcrossingtechnique. Backcrossing methods can be used with the present inventionto improve or introduce a characteristic into the variety. The term“backcrossing” as used herein refers to the repeated crossing of ahybrid progeny back to the recurrent parent, i.e., backcrossing 1, 2, 3,4, 5, 6, 7, 8, 9, or more times to the recurrent parent. The parentalcotton plant which contributes the gene for the desired characteristicis termed the “nonrecurrent” or “donor parent”. This terminology refersto the fact that the nonrecurrent parent is used one time in thebackcross protocol and therefore does not recur. The parental cottonplant to which the gene or genes from the nonrecurrent parent aretransferred is known as the recurrent parent as it is used for severalrounds in the backcrossing protocol (Poehlman & Sleper (1994); Fehr(1987)). In a typical backcross protocol, the original variety ofinterest (recurrent parent) is crossed to a second variety (nonrecurrentparent) that carries the single gene of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a cotton plant isobtained wherein essentially all of the desired morphological andphysiological characteristics of the recurrent parent are recovered inthe converted plant, in addition to the single transferred gene from thenonrecurrent parent, as determined at the 5% significance level whengrown in the same environmental conditions.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single gene of the recurrent variety ismodified or substituted with the desired gene from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphological,constitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross. One ofthe major purposes is to add some commercially desirable, agronomicallyimportant trait to the plant. The exact backcrossing protocol willdepend on the characteristic or trait being altered to determine anappropriate testing protocol. Although backcrossing methods aresimplified when the characteristic being transferred is a dominantallele, a recessive allele may also be transferred. In this instance itmay be necessary to introduce a test of the progeny to determine if thedesired characteristic has been successfully transferred.

Many single gene traits have been identified that are not regularlyselected for in the development of a new variety but that can beimproved by backcrossing techniques. Single gene traits may or may notbe transgenic. Examples of these traits include but are not limited to,male sterility, waxy starch, herbicide resistance, resistance forbacterial, fungal, or viral disease, insect resistance, male fertility,enhanced nutritional quality, industrial usage, yield stability, andyield enhancement. These genes are generally inherited through thenucleus. Several of these single gene traits are described in U.S. Pat.Nos. 5,959,185; 5,973,234; and 5,977,445, the disclosures of which arespecifically hereby incorporated by reference.

Further reproduction of the variety can occur by tissue culture andregeneration. Tissue culture of various tissues of cotton andregeneration of plants therefrom is well known and widely published. Forexample, reference may be had to Komatsuda, T., et al., Crop Sci.,31:333-337 (1991); Stephens, P. A., et al., Theor. Appl. Genet.,82:633-635 (1991); Komatsuda, T., et al., Plant Cell, Tissue and OrganCulture, 28:103-113 (1992); Dhir, S., et al. Plant Cell Rep., 11:285-289(1992); Pandey, P., et al., Japan J. Breed., 42:1-5 (1992); and Shetty,K., et al., Plant Science, 81:245-251 (1992); as well as U.S. Pat. No.5,024,944 issued Jun. 18, 1991 to Collins, et al., and U.S. Pat. No.5,008,200 issued Apr. 16, 1991 to Ranch, et al. Thus, another aspect ofthis invention is to provide cells which upon growth and differentiationproduce cotton plants having the physiological and morphologicalcharacteristics of cotton cultivar L-9009-6.

As used herein, the term “tissue culture” indicates a compositioncomprising isolated cells of the same or a different type or acollection of such cells organized into parts of a plant. Exemplarytypes of tissue cultures are protoplasts, calli, plant clumps, and plantcells that can generate tissue culture that are intact in plants orparts of plants, such as embryos, pollen, flowers, seeds, leaves, stems,roots, root tips, anthers, pistils, and the like. Means for preparingand maintaining plant tissue culture are well known in the art. By wayof example, a tissue culture comprising organs has been used to produceregenerated plants. U.S. Pat. Nos. 5,959,185; 5,973,234; and 5,977,445,described certain techniques.

This invention also is directed to methods for producing a cotton plantby crossing a first parent cotton plant with a second parent cottonplant wherein the first or second parent cotton plant is a cotton plantof the cultivar L-9009-6. Further, both first and second parent cottonplants can come from the cotton cultivar L-9009-6. Additionally, thefirst or second parent cotton plants can be either Gossypium hirsutum orGossypium barbadense, or any other cotton plant. Thus, any such methodsusing the cotton cultivar L-9009-6 are part of this invention: selfing,backcrosses, hybrid production, crosses to populations, and the like.All plants produced using cotton cultivar L-9009-6 as a parent arewithin the scope of this invention, including those developed fromvarieties derived from cotton cultivar L-9009-6. Advantageously, thecotton cultivar could be used in crosses with other, different, cottonplants to produce first generation (F₁) cotton hybrid seeds and plantswith superior characteristics. The other, different, cotton plants maybe Gossypium hirsutum or Gossypium barbadense or another cottoncultivar. The cultivar of the invention can also be used fortransformation where exogenous genes are introduced and expressed by thecultivar of the invention. Genetic variants created either throughtraditional breeding methods using cultivar L-9009-6 or throughtransformation of L-9009-6 by any of a number of protocols known tothose of skill in the art are intended to be within the scope of thisinvention.

The following describes breeding methods that may be used with cultivarL-9009-6 in the development of further cotton plants. One suchembodiment is a method for developing a L-9009-6 progeny cotton plant ina cotton plant breeding program comprising: obtaining the cotton plant,or a part thereof, of cultivar L-9009-6, utilizing said plant or plantpart as a source of breeding material, and selecting a L-9009-6 progenyplant with molecular markers in common with L-9009-6 and/or withmorphological and/or physiological characteristics selected from thecharacteristics listed in Tables 1, 2, 3 or 4. Breeding steps that maybe used in the cotton plant breeding program include pedigree breeding,backcrossing, mutation breeding, and recurrent selection. In conjunctionwith these steps, techniques such as RFLP-enhanced selection, geneticmarker enhanced selection (for example, SSR markers), and the making ofdouble haploids may be utilized.

Another method involves producing a population of cultivar L-9009-6progeny cotton plants, comprising crossing cultivar L-9009-6 withanother cotton plant, thereby producing a population of cotton plants,which, on average, derive 50% of their alleles from cultivar L-9009-6.The other cotton plant may be Gossypium hirsutum or Gossypium barbadenseor any other cotton plant. A plant of this population may be selectedand repeatedly selfed or sibbed with a cotton cultivar resulting fromthese successive filial generations. One embodiment of this invention isthe cotton cultivar produced by this method and that has obtained atleast 50% of its alleles from cultivar L-9009-6.

One of ordinary skill in the art of plant breeding would know how toevaluate the traits of two plant varieties to determine if there is nosignificant difference between the two traits expressed by thosevarieties. For example, see Fehr and Walt, Principles of CultivarDevelopment, pp. 261-286 (1987). Thus the invention includes cottoncultivar L-9009-6 progeny cotton plants comprising a combination of atleast two L-9009-6 traits selected from the group consisting of thoselisted in Tables 1, 2, 3 or 4 or the L-9009-6 combination of traitslisted in the Summary of the Invention, so that said progeny cottonplant is not significantly different for said traits than cottoncultivar L-9009-6 as determined at the 5% significance level when grownin the same environment. Using techniques described herein, molecularmarkers may be used to identify said progeny plant as a L-9009-6 progenyplant. Mean trait values may be used to determine whether traitdifferences are significant, and preferably the traits are measured onplants grown under the same environmental conditions. Once such avariety is developed its value is substantial since it is important toadvance the germplasm base as a whole in order to maintain or improvetraits such as yield, disease resistance, pest resistance, and plantperformance in extreme environmental conditions.

Progeny of cultivar L-9009-6 may also be characterized through theirfilial relationship with cotton cultivar L-9009-6, as for example, beingwithin a certain number of breeding crosses of cotton cultivar L-9009-6.A breeding cross is a cross made to introduce new genetics into theprogeny, and is distinguished from a cross, such as a self or a sibcross, made to select among existing genetic alleles. The lower thenumber of breeding crosses in the pedigree, the closer the relationshipbetween cotton cultivar L-9009-6 and its progeny. For example, progenyproduced by the methods described herein may be within 1, 2, 3, 4 or 5breeding crosses of cotton cultivar L-9009-6.

As used herein, the term “plant” includes plant cells, plantprotoplasts, plant cell tissue cultures from which cotton plants can beregenerated, plant calli, plant clumps, and plant cells that are intactin plants or parts of plants, such as embryos, pollen, ovules, flowers,leaves, roots, root tips, anthers, pistils, and the like.

Deposit Information

A deposit of the cotton variety named L-9009-6 disclosed above andrecited in the appended claims has been made with the NationalCollections of Industrial, Food and Marine Bacteria (NCIMB), NCIMB Ltd.,Ferguson Building, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA,Scotland, UK. The date of deposit was Oct. 13, 2010. The deposit of2,500 seeds was taken from the same deposit maintained by Spirou Groupof Companies since prior to the filing date of this application. Allrestrictions upon the deposit have been removed, and the deposit isintended to meet all of the requirements of 37 C.F.R. §1.801-1.809. TheNCIMB accession number is NCIMB No. 41770. The deposit will bemaintained in the depository for a period of 30 years, or 5 years afterthe last request, or for the effective life of the patent, whichever islonger, and will be replaced as necessary during that period.

While a number of exemplary aspects and embodiments have been discussedabove, those of skill in the art will recognize certain modifications,permutations, additions, and sub-combinations thereof. It is thereforeintended that the following appended claims and claims hereafter areinterpreted to include all such modifications, permutations, additions,and sub-combinations as are within their true spirit and scope.

1. A seed of cotton cultivar L-9009-6, wherein a representative sampleof seed of said cultivar was deposited under NCIMB No.
 41770. 2. Acotton plant, or a part thereof, produced by growing the seed ofclaim
 1. 3. A tissue culture of cells produced from the plant of claim2, wherein said cells of the tissue culture are produced from a plantpart selected from the group consisting of leaves, pollen, embryos,cotyledons, hypocotyl, meristematic cells, roots, root tips, pistils,anthers, flowers, and stems.
 4. A protoplast produced from the plant ofclaim
 2. 5. A protoplast produced from the tissue culture of claim
 3. 6.A cotton plant regenerated from the tissue culture of claim 3, whereinthe plant has the morphological and physiological characteristics ofcultivar L-9009-6 listed in Table 1, wherein a representative sample ofseed was deposited under NCIMB No.
 41770. 7. A method for producing anF₁ hybrid cotton seed, wherein the method comprises crossing the plantof claim 2 with a different cotton plant and harvesting the resultant F₁hybrid cotton seed.
 8. A hybrid cotton seed produced by the method ofclaim
 7. 9. A hybrid cotton plant, or a part thereof, produced bygrowing said hybrid seed of claim
 8. 10. A method of producing anherbicide resistant cotton plant, wherein the method comprisestransforming the cotton plant of claim 2 with a transgene wherein thetransgene confers resistance to an herbicide selected from the groupconsisting of imidazolinone, sulfonylurea, glyphosate, glufosinate,L-phosphinothricin, triazine and benzonitrile.
 11. An herbicideresistant cotton plant produced by the method of claim
 10. 12. A methodof producing an insect resistant cotton plant, wherein the methodcomprises transforming the cotton plant of claim 2 with a transgene thatconfers insect resistance.
 13. An insect resistant cotton plant producedby the method of claim
 12. 14. The cotton plant of claim 13, wherein thetransgene encodes a Bacillus thuringiensis endotoxin.
 15. A method ofproducing a disease resistant cotton plant, wherein the method comprisestransforming the cotton plant of claim 2 with a transgene that confersdisease resistance.
 16. A disease resistant cotton plant produced by themethod of claim
 15. 17. A method of producing a cotton plant withmodified fatty acid metabolism or modified carbohydrate metabolism,wherein the method comprises transforming the cotton plant of claim 2with a transgene encoding a protein selected from the group consistingof phytase, fructosyltransferase, levansucrase, α-amylase, invertase andstarch branching enzyme or encoding an antisense of stearyl-ACPdesaturase.
 18. A cotton plant having modified fatty acid metabolism ormodified carbohydrate metabolism produced by the method of claim
 17. 19.A method of introducing a desired trait into cotton cultivar L-9009-6,wherein the method comprises: (a) crossing a L-9009-6 plant, wherein arepresentative sample of seed was deposited under NCIMB No. 41770, witha plant of another cotton cultivar that comprises a desired trait toproduce progeny plants wherein the desired trait is selected from thegroup consisting of male sterility, herbicide resistance, insectresistance, modified fatty acid metabolism, modified carbohydratemetabolism and resistance to bacterial disease, fungal disease or viraldisease; (b) selecting one or more progeny plants that have the desiredtrait to produce selected progeny plants; (c) crossing the selectedprogeny plants with the L-9009-6 plants to produce backcross progenyplants; (d) selecting for backcross progeny plants that have the desiredtrait and the physiological and morphological characteristics of cottoncultivar L-9009-6 listed in Table 1 to produce selected backcrossprogeny plants; and (e) repeating steps (c) and (d) two or more times insuccession to produce selected fourth or higher backcross progeny plantsthat comprise the desired trait and the physiological and morphologicalcharacteristics of cotton cultivar L-9009-6 listed in Table
 1. 20. Acotton plant produced by the method of claim 19, wherein the plant hasthe desired trait and the physiological and morphologicalcharacteristics of cotton cultivar L-9009-6 listed in Table
 1. 21. Thecotton plant of claim 20, wherein the desired trait is herbicideresistance and the resistance is conferred to an herbicide selected fromthe group consisting of imidazolinone, sulfonylurea, glyphosate,glufosinate, L-phosphinothricin, triazine and benzonitrile.
 22. Thecotton plant of claim 20, wherein the desired trait is insect resistanceand the insect resistance is conferred by a transgene encoding aBacillus thuringiensis endotoxin.
 23. The cotton plant of claim 20,wherein the desired trait is modified fatty acid metabolism or modifiedcarbohydrate metabolism and said desired trait is conferred by a nucleicacid encoding a protein selected from the group consisting of phytase,fructosyltransferase, levansucrase, α-amylase, invertase and starchbranching enzyme or encoding an antisense of stearyl-ACP desaturase.